Coding

Part:BBa_K2074022:Design

Designed by: Yi Cai   Group: iGEM16_FAFU-CHINA   (2016-10-09)


Cry10Aa(Codon optimization)-Extended FMDV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
    Illegal NgoMIV site found at 223
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 784
    Illegal NgoMIV site found at 1460
    Illegal NgoMIV site found at 2116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 202
    Illegal SapI.rc site found at 924


Design Notes

To identify there is no restriction enzyme cutting site which we choosed.


Source

DNA from a synthesis company.

References